CST, 56440SF, KCC2 (D1R2R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying KCC2. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, KCC2 (D1R2R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #56440) is ready to ship. Product Usage Information This product is the carrier free version of product #94725. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 94725 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity KCC2 (D1R2R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total KCC2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala970 of human KCC2 protein. Background The potassium/chloride cotransporter 2 (KCC2, SLC12A5) is a neuron-specific transport protein responsible for regulating the cotransport of potassium and chloride ions. KCC2 uses the energy of the electrochemical potassium gradient to export chloride ions from cells, therefore maintaining intracellular chloride ion concentrations in mature neurons (1,2). The intracellular concentration of chloride ions determines the neuronal response to the inhibitory neurotransmitter GABA and glycine. As a result, KCC2 can play a critical role in regulating neuronal excitability in mature central nervous system neurons (3-5). Altered KCC2 expression and reduced KCC2 activity can result in an increase in intracellular chloride ion concentrations and subsequent hyperexcitability of neuronal systems. Cases of aberrant KCC2 function are associated with neurological disorders, such as multiple forms of epilepsy, neuropathic pain, and schizophrenia (6-10). Alternate Names EIEE34; EIG14; Electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; hKCC2; K-Cl cotransporter 2; KCC2; KIAA1176; Neuronal K-Cl cotransporter; S12A5; SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; Solute carrier family 12 member 5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130-260 Source/Isotype: Rabbit IgG