CST, 5645S, Phospho-TACC3 (Ser558) Antibody

Polyclonal Antibody for studying TACC3 (Ser558) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-TACC3 (Ser558) Antibody (CST #5645) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-TACC3 (Ser558) Antibody detects endogenous levels of TACC3 protein only when phosphorylated at Ser558. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser558 of human TACC3 protein. Antibodies are purified by Protein A and peptide affinity chromatography. Background Transforming acid coiled-coil (TACC) proteins are a family of proteins characterized by a common coiled-coil motif of approximately 200 amino acids at the carboxy-terminal end (1). Three family members have been identified in humans: TACC1, TACC2, and TACC3. These proteins are thought to be involved in centrosomal microtubule assembly and have been mapped to chromosomal regions that are disrupted in some cancers (reviewed in 2). TACC3 has been shown to be upregulated in many cancer cell lines (3). When phosphorylated at Ser558 by Aurora A, mammalian TACC3 is localized to mitotic spindles and increases microtubule stability (4,5). For this reason, it has been suggested that monitoring the localization of phosphorylated TACC3 would be an effective way to determine the efficacy of Aurora A inhibitors that show promise as anti-cancer drugs (6,7). In addition, studies have shown that TACC3 could be useful as a prognostic marker for non-small cell lung cancer (8). Alternate Names ERIC-1; ERIC1; maskin; MGC117382; MGC133242; TACC3; Tacc4; transforming acidic coiled-coil containing protein 3; Transforming acidic coiled-coil-containing protein 3; transforming, acidic coiled-coil containing protein 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 SOURCE: Rabbit