CST, 5648T, CA9 (D10C10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CA9. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CA9 (D10C10) Rabbit Monoclonal Antibody (CST #5648) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 83980 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CA9 (D10C10) Rabbit Monoclonal Antibody recognizes endogenous level of total CA9 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp361 of human CA9 protein. Background Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO into HCO and H and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2). CA9 is a member of alpha class, a plasma membrane protein with the catalytic domain in the extracellular space. Its expression is restricted to very few normal tissues (mainly the gastrointestinal tract) (2). CA9 expression is strongly induced by hypoxia and down-regulated by the wildtype von Hippel-Lindau (VHL) tumor suppressor protein. CA9 expression is increased in many types of tumor, especially in solid hypoxic tumors with a poor responsiveness to the conventional radio-and/or chemo-therapies; CA9 is considered as a tumor hypoxia marker and a promising target for cancer therapeutic intervention (3-5). Alternate Names CA-IX; CA9; CAH9; CAIX; Car9; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35-58 Source/Isotype: Rabbit IgG