CST, 56500S, GSK-3 beta (27C10) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying GSK3-beta. Validated for Western Blotting. Highly specific and rigorously validated in-house, GSK-3 beta (27C10) Rabbit Monoclonal Antibody (Biotinylated) (CST #56500) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GSK-3 beta (27C10) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of total GSK-3β protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GSK-3β protein. Background Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in and is a component of the Wnt signaling pathway required for and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Alternate Names glycogen synthase kinase 3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta; GSK3beta isoform; Serine/threonine-protein kinase GSK3B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 46 Source/Isotype: Rabbit IgG