CST, 56501S, WTAP Antibody

Polyclonal Antibody for studying WTAP. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, WTAP Antibody (CST #56501) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity WTAP Antibody recognizes endogenous levels of total WTAP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human WTAP protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Methyltransferase-like protein 3 (METTL3) and methyltransferase-like protein 14 (METTL14) are the two catalytic subunits of an N6-methyltransferase complex that methylates adenosine residues in RNA (1). Methylation of adenosine residues regulates mRNA splicing, processing, translation efficiency, editing and stability, in addition to regulating primary miRNA processing, and is critical for proper regulation of the circadian clock, embryonic stem cell self-renewal, immune tolerance, response to various stimuli, meiosis and mouse fertility (2,3). In this complex, METTL3 functions as the catalytic methyltransferase subunit and METTL14 functions as the target recognition subunit by binding to RNA (4). In addition, the Wilms' tumor 1-associating protein (WTAP) functions as a regulatory subunit and is required for accumulation of the complex to nuclear speckles, which are sites of RNA processing (5). Several studies suggest a role for this complex in cancer. METTL3 expression is elevated in lung adenocarcinoma where it promotes growth, survival, and invasion of human lung cancer cells (6). In addition, WTAP is overexpressed in a number of different cancers and positively regulates cell migration and invasion in glioblastoma and cholangiocarcinoma (7,8). Alternate Names DKFZp686F20131; Female-lethal(2)D homolog; FL2D; hFL(2)D; KIAA0105; MGC3925; Mum2; PNAS-132; Pre-mRNA-splicing regulator WTAP; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; Wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; WT1 associated protein; WT1-associated protein; WTAP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit