CST, 56538S, IL4R (F6C4Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL4R mouse. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, IL4R (F6C4Z) Rabbit Monoclonal Antibody (CST #56538) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37775 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IL4R (F6C4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL4R protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IL4R protein. Background In the immune system, interleukin-4 (IL-4) controls B cell growth, survival, and maturation, in addition to proliferation and differentiation of the Th2 subset of T cells. IL-4 is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). It acts through the IL-4 receptor (IL4R), leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (3). IL4R is a transmembrane ligand binding protein composed of a heterodimer alpha and gamma receptor complex (4,5). IL4R has been shown to bind both IL-4 and interleukin-13 (IL-13), regulating immunoglobulin E (IgE) and chemokine production during allergic inflammation (6). In addition to its role in regulating immune response, signaling through IL4R is also implicated in diseases such as asthma, allergy, and cancer, making it a potent target for various therapies (7-9). Increased IL4R expression has been observed in tumors (9,10), and several mutations in IL4R are associated with metastatic cancers, underscoring its importance in cancer progression and as a target for chemotherapy development (10,11). Alternate Names CD124; IL-4 receptor alpha chain; IL-4 receptor subunit alpha; IL-4-binding protein; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; IL4-BP; Il4r; Il4ra; interleukin 4 receptor, alpha; Interleukin-4 receptor subunit alpha; MGC118473; sIL4Ralpha/prot; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; Soluble interleukin-4 receptor subunit alpha Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 75-80, 120 Source/Isotype: Rabbit IgG