CST, 56548SF, VISTA (D5L5T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying VISTA mouse. Validated for WB,IHC,IHC,IF,IF. Highly specific and rigorously validated in-house, VISTA (D5L5T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #56548) is ready to ship. Product Usage Information This product is the carrier free version of product #54979. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 54979 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity VISTA (D5L5T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total VISTA protein. This antibody is not recommended for immunohistochemical analysis of human tissues. Instead, VISTA (D1L2G) Rabbit Monoclonal Antibody #64953 is suggested. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val291 of mouse VISTA protein. Background VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7). Alternate Names 4632428N05Rik; Die; Dies1; differentiation of ESCs 1; GI24; Gi24 receptor; PD-1; PD-1H; Platelet receptor Gi24; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation; VISTA; Vsir Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45-65 Source/Isotype: Rabbit IgG