CST, 5657S, RAIG1 Antibody

Polyclonal Antibody for studying RAIG1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, RAIG1 Antibody (CST #5657) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RAIG1 Antibody recognizes endogenous levels of total RAIG1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val295 of human RAIG1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Retinoic Acid-Induced Gene 1 (RAIG1), also called RAI3 and GPRC5A, belongs to the family of G protein-coupled receptors (GPCRs). GPCRs are the largest and most abundant receptor family in mammals, composed of more than one thousand members (1). Retinoic acids (RA) are a group of pleiotropic signaling molecules that regulate a broad range of physiologic and developmental processes. It is thought that RAIG1 may be a downstream target of RA signaling (2). RAIG1 is expressed in lung tissue and in several lung cancer cell lines, and encodes a seven-transmembrane glycoprotein presumed to be a lung tumor suppressor gene (3,4). Alternate Names G protein-coupled receptor class C group 5 member A; G protein-coupled receptor, family C, group 5, member A; G-protein coupled receptor family C group 5 member A; GPCR5A; GPRC5A; Orphan G-protein-coupling receptor PEIG-1; PEIG-1; Phorbol ester induced gene 1; phorbol ester induced protein-1; RAI3; RAIG-1; RAIG1; retinoic acid induced 3; retinoic acid responsive; Retinoic acid-induced gene 1 protein; Retinoic acid-induced protein 3; TIG1; TPA induced gene 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 SOURCE: Rabbit