CST, 5659T, Non-phospho-STEP (Ser221) (D74H3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STEP (Ser245) non-phosphate. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Non-phospho-STEP (Ser221) (D74H3) Rabbit Monoclonal Antibody (CST #5659) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity Non-phospho-STEP (Ser221) (D74H3) Rabbit Monoclonal Antibody detects endogenous levels of STEP61 protein only when dephosphorylated at Ser221 and of STEP46 protein when dephosphorylated at Ser49. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic nonphosphopeptide corresponding to residues surrounding Ser221 of mouse STEP61 protein. Background Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer's disease (1). Activity of STEP61 is reduced upon phosphorylation of Ser221 (Ser49 of STEP46) due to lower affinity for its substrates (2). Alternate Names FLJ14427; Neural-specific protein-tyrosine phosphatase; protein tyrosine phosphatase non-receptor type 5; protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched); protein-tyrosine phosphatase striatum-enriched; PTN5; PTPN5; PTPSTEP; STEP; STEP61; striatal-enriched protein tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase; Tyrosine-protein phosphatase non-receptor type 5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 46, 61 Source/Isotype: Rabbit IgG