CST, 56601W2, CD133 (A8N6N) Mouse Monoclonal Antibody (SignalFlex™ Alexa Fluor® 750 Conjugate)

Monoclonal Antibody for studying CD133. Highly specific and rigorously validated in-house, CD133 (A8N6N) Mouse Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 750 Conjugate) (CST #56601) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity CD133 (A8N6N) Mouse mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with cells overexpressing human CD133 protein. Background CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8). Alternate Names AC133; Antigen AC133; CD133; CORD12; hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; PROM1; prominin 1; Prominin-1; prominin-like 1; Prominin-like protein 1; PROML1; RP41; STGD4 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG3