CST, 56616T, REV1 (F6R6I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rev1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, REV1 (F6R6I) Rabbit Monoclonal Antibody (CST #56616) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity REV1 (F6R6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total REV1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human REV1 protein. Background Translesion synthesis (TLS) is a DNA damage tolerance mechanism that allows error-prone DNA polymerases to bypass lesions that would otherwise block DNA replication. This bypass prevents replication fork collapse, avoiding genome instability and potentially tumorigenesis. TLS requires specialized DNA polymerases, which in humans include the Y-family polymerases POLη, POLι, POLκ, and REV1, as well as the B-family polymerase POLζ/REV3L (1,2). Targeting TLS is a promising therapeutic strategy in multiple cancers (3). REV1 is critical for the recruitment and assembly of TLS polymerases at DNA damage sites. The C-terminal domain (CTD) of REV1 is essential for its function, making it a potential therapeutic target (4). Expression of the REV1 CTD has a dominant negative effect on TLS, sensitizing cancer cells to DNA-damaging agents (5). Additional REV1 inhibitors have been shown to sensitize cancer cells to radiation and chemotherapy (6-8). Alternate Names AIBP80; Alpha integrin-binding protein 80; DNA repair protein REV1; FLJ21523; MGC163283; MGC26225; REV1; REV1 DNA directed polymerase; REV1 homolog; REV1 homolog (S. cerevisiae); REV1- like; Rev1-like terminal deoxycytidyl transferase; REV1, polymerase (DNA directed); REV1L Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG