CST, 5662S, PDK1 (D37A7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDK1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PDK1 (D37A7) Rabbit Monoclonal Antibody (CST #5662) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PDK1 (D37A7) Rabbit Monoclonal Antibody detects endogenous levels of total PDK1 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PDK1 protein. Background Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis. Alternate Names 3-phosphoinositide dependent protein kinase 1; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; hPDK1; MGC20087; MGC35290; PDK1; PDPK1; PDPK2; PDPK2P; PkB kinase like gene 1; PkB-like 1; PRO0461; protein kinase Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 58-68 Source/Isotype: Rabbit IgG