CST, 56654T, UPF3B (E5U4C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UPF3B. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, UPF3B (E5U4C) Rabbit Monoclonal Antibody (CST #56654) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity UPF3B (E5U4C) Rabbit Monoclonal Antibody recognizes endogenous levels of total UPF3B protein. This antibody does not detect UPF3A. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu262 of human UPF3B protein. Background UPF3A and UPF3B are paralogs derived from a gene duplication and are involved in nonsense-mediated decay (NMD), a process that eliminates aberrantly formed mRNAs (1,2). While UPF3A seems to be an NMD repressor, UPF3B serves as an NMD activator, by binding to the exon junction complex (EJC) and shuttling to the cytoplasm after mRNA export (2-4). Under normal conditions, UPF3B will outcompete UPF3A binding to the EJC and key NMD factor UPF2, but upon UPF3B loss, UPF3A is upregulated and can become an NMD activator (4,5). UPF3 activation of NMD, however, can be independent of EJC binding (6). Though UPF3B is dispensable for global NMD, certain transcripts rely on it, suggesting there is both a UPF3B-dependent and independent pathway (7,8). UPF3B also plays a role in translation termination by causing dissociation of ribosomal complexes bound to mRNAs with premature termination codons (9). UPF3B can regulate differentiation of neural stem cells, and mutations and aberrant expression has been described in various neurological disorders, including autism and ALS (10-13). Alternate Names hUpf3B; hUpf3p-X; mental retardation, X-linked 62; MRX62; MRX82; MRXS14; nonfunctional UPF3B regulator of nonsense mediated mRNA decay; Nonsense mRNA reducing factor 3B; Regulator of nonsense transcripts 3B; REN3B; RENT3B; Up-frameshift suppressor 3 homolog B; Up-frameshift suppressor 3 homolog on chromosome X; UPF3 regulator of nonsense transcripts homolog B; UPF3 regulator of nonsense transcripts homolog B (yeast); UPF3B; UPF3B pseudogene 1; UPF3B pseudogene 2; UPF3B pseudogene 3; UPF3B regulator of nonsense mediated mRNA decay; UPF3BP1; UPF3BP2; UPF3BP3; Upf3p-X; UPF3X Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG