CST, 56658SF, HSP60 (D6F1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying HSP60. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, HSP60 (D6F1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #56658) is ready to ship. Product Usage Information This product is the carrier free version of product #12165. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12165 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity HSP60 (D6F1) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total HSP60 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Xenopus, Zebrafish, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp68 of human HSP60 protein. Background In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8). Alternate Names 60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; CH60; Chaperonin 60; CPN60; epididymis secretory sperm binding protein; GROEL; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; heat shock protein family D (Hsp60) member 1; HLD4; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13 Specification REACTIVITY: H M R Hm Mk X Z B Pg SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG