CST, 5666S, beta3-Tubulin (D65A4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying tubulin beta-3. Validated for WB,WB,IP,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, beta3-Tubulin (D65A4) Rabbit Monoclonal Antibody (CST #5666) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 44456 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity beta3-Tubulin (D65A4) Rabbit Monoclonal Antibody detects endogenous levels of total β3-tubulin protein. This antibody does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein. Background The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). β3-tubulin (TUBB3) is one of six β-tubulin isoforms and is expressed highly during fetal and postnatal development (axon guidance and maturation) (2). Its expression levels decrease in the adult central nervous system (CNS) but remain high in the peripheral nervous system (PNS) (3). Microtubules enriched in β3-tubulin are more dynamic than those composed of other β-tubulin isoforms (4). Research studies have shown that mutations in the β3-tubulin gene cause ocular motility defects and other nervous system disorders. Furthermore, β3-tubulin is present in neoplastic but not in normal differentiated glial cells. Thus, β3-tubulin is a great neuronal marker (5). Alternate Names Beta III Tubulin; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; class III beta-tubulin; FEOM3; MC1R; TBB3; TUBB3; TUBB4; tubulin beta 3 class III; Tubulin beta-3 chain; tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3; tubulin, beta, 4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG