CST, 56678T, GPR75 (F3P5Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GPR75. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GPR75 (F3P5Y) Rabbit Monoclonal Antibody (CST #56678) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GPR75 (F3P5Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total GPR75 protein. This antibody detects a 50 kDa protein of unknown identity in some tissues. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala430 of human GPR75 protein. Background GPR75 is a G protein-coupled receptor. Protein-truncating variants were associated with leanness and protection against obesity in humans. In addition, studies show that knockout (KO) mice fed with a high-fat diet are resistant to weight gain and have improved glycemic control and insulin sensitivity compared with wild-type (WT) mice (1). Furthermore, studies show that, in mice, mRNA expression is the most abundant in the brain. is widely expressed in the mouse brain, and a subset of is co-expressed with appetite-regulating hypothalamic neurons. KO mice are protected against liver lipid accumulation induced by a high-fat diet compared with WT mice (2). Moreover, studies on KO mice suggest a role of Gpr75 in the development of hypoxia-induced pulmonary hypertension (3). Alternate Names G protein-coupled receptor 75; GPR75; GPRchr2; MGC132002; Probable G-protein coupled receptor 75; WI31133 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG