CST, 56687S, p63-alpha (D2K8X) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying p63. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, p63-alpha (D2K8X) Rabbit Monoclonal Antibody (PE Conjugate) (CST #56687) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity p63-alpha (D2K8X) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total p63-α protein. This antibody conjugate will detect both the full-length isoform (TAp63-α) and the truncated δNp63-α isoform, but will not recognize the p63-β or p63-γ isoform. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p63-α protein. Background The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a truncated ΔNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4). Alternate Names AIS; amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; DeltaNp63; EEC3; Keratinocyte transcription factor KET; KET; LMS; NBP; OFC8; p40; p51; p53CP; P63; P73H; P73L; RHS; SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; Tumor protein 63; tumor protein p53-competing protein; tumor protein p53-like; tumor protein p63; tumor protein p63 deltaN isoform delta; Tumor protein p73-like Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG