CST, 5668S, STIM1 (D88E10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STIM1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, STIM1 (D88E10) Rabbit Monoclonal Antibody (CST #5668) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity STIM1 (D88E10) Rabbit Monoclonal Antibody recognizes endogenous levels of total STIM1 protein. This antibody does not cross-react with STIM2. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro622 of human STIM1 protein. Background Ca is a key second messenger in many intracellular signaling pathways. Ca signals control many cellular functions ranging from short-term responses such as contraction and secretion to longer-term regulation of cell growth and proliferation (1,2). Stromal interaction molecules (STIMs) function as Ca sensors that detect changes in Ca content in intracellular Ca stores (3). STIM1 is conserved, ubiquitously expressed, and functions as an endoplasmic reticulum (ER) Ca sensor that migrates from the ER Ca store to the plasma membrane where it activates calcium-release-activated calcium (CRAC) channels when the ER Ca store is low (4). STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors (5). STIM1 can either homodimerize or form heterodimers with STIM2. STIM2 possesses a high sequence identity to STIM1 and can function as an inhibitor of STIM1-mediated plasma membrane store-operated Ca entry (6). However, further investigation is required to elucidate the true physiological function of STIM2. Alternate Names D11S4896E; GOK; IMD10; STIM1; STRMK; Stromal interaction molecule 1; TAM; TAM1 Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG