CST, 5670S, HOP (D10E2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HOP. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, HOP (D10E2) Rabbit Monoclonal Antibody (CST #5670) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 20138 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HOP (D10E2) Rabbit Monoclonal Antibody recognizes endogenous levels of total HOP protein. This antibody is not known to cross-react with other heat shock proteins. Species Reactivity: Human, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro391 of human HOP protein. Background HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5). Alternate Names epididymis secretory sperm binding protein Li 94n; HEL-S-94n; Hop; Hsc70/Hsp90-organizing protein; Hsp70/Hsp90-organizing protein; IEF-SSP-3521; NY-REN-11 antigen; P60; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein); Transformation-sensitive protein IEF SSP 3521 Specification REACTIVITY: H Mk B SENSITIVITY: Endogenous MW (kDa): 64 Source/Isotype: Rabbit IgG