CST, 56712S, GM-CSF (E8I1V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GMCSF. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GM-CSF (E8I1V) Rabbit Monoclonal Antibody (CST #56712) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GM-CSF (E8I1V) Rabbit Monoclonal Antibody recognizes endogenous levels of total GM-CSF protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro29 of human GM-CSF protein. Background GM-CSF is produced by activated T cells, NK cells and macrophages (1,5). Target cells include granulocyte, monocyte precursors and subsets of differentiated myeloid cells (1,2,3). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in hematopoietic differentiation of dendritic cells, and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (4). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5, and PI3K/Akt pathways (1). Alternate Names colony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GM-CSF; GMCSF; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; molgramostim; Molgramostin; Sargramostim Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15, 18, 22 Source/Isotype: Rabbit IgG