CST, 56719S, NALP1 (E3F2U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NALP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NALP1 (E3F2U) Rabbit Monoclonal Antibody (CST #56719) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NALP1 (E3F2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total NALP1 protein. This antibody recognizes both the 165 kDa full-length NALP1 and the auto-processed 27 kDa C-terminal fragment. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human NALP1 protein. Background NALP1 (DEFCAP/NAC/CARD7) is an NLR (Nod-like receptor) family member that has been implicated in the regulation of apoptosis and inflammatory responses (1-5). Structurally, NALP contains an amino-terminal PYRIN domain, followed by a nucleotide-binding site (NBS), a leucine-rich repeat region (LRR), and a carboxy-terminal CARD domain. NALP1 interacts strongly with caspase-2 and weakly with caspase-9, and induces apoptosis when overexpressed (3). Similar to a related Ced-4 family member Apaf-1, it was also shown to be involved in cytochrome c-dependent caspase activation (2). It has also been shown to be part of the "inflammasome" comprised of caspase-1, caspase-5, and Pycard/ASC, which is critical in the processing of pro-inflammatory cytokines like IL-1β (6). Two major isoforms were identified for NALP1, which differ in a 44 amino acid region within the LRR (3). In addition, like NALP3, a short NALP1 isoform lacking the LRR (NALP1 ) likely exists (7). Polymorphisms in NALP1 have been associated with autoimmune diseases (8) and susceptibility to toxins (9). NALP1 is autoproteolytically processed into a large N-terminal and a small C-terminal fragment, which are non-covalently associated. Upon activation, the C-terminal is released and forms inflammasomes with other proteins (10-12). Alternate Names AIADK; CARD7; caspase recruitment domain protein 7; Caspase recruitment domain-containing protein 7; CIDED; CLR17.1; Death effector filament-forming ced-4-like apoptosis protein; DEFCAP; DEFCAP-L/S; DKFZp586O1822; JRRP; KIAA0926; MSPC; NAC; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; NACHT, leucine rich repeat and PYD containing 1; NACHT, LRR and PYD containing protein 1; NACHT, LRR and PYD domains-containing protein 1; NALP1; NLR family pyrin domain containing 1; NLR family, pyrin domain containing 1; NLRP1; Nucleotide-binding domain and caspase recruitment domain; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1; PP1044; SLEV1; VAMAS1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 165 Source/Isotype: Rabbit IgG