CST, 5675T, PKA RI-alpha (D54D9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRKAR1A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PKA RI-alpha (D54D9) Rabbit Monoclonal Antibody (CST #5675) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PKA RI-alpha (D54D9) Rabbit Monoclonal Antibody recognizes endogenous levels of total PKA RI-α protein. This antibody may also detect PKA RI-β and detects a background band of unknown origin at 32 kDa. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln371 of human PKA RI-α protein. Background The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β (RI-α, RI-β, RII-α, and RII-β). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue (2). Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK-3β Ser9) (3-5). In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259) (6). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197 (7). Alternate Names ACRDYS1; ADOHR; cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; CAR; Carney complex type 1; CNC; CNC1; DKFZp779L0468; epididymis secretory sperm binding protein; KAP0; MGC17251; PKAR1A; PKR1; PPNAD1; PRKAR1; PRKAR1A; protein kinase A type 1a regulatory subunit; protein kinase cAMP-dependent type I regulatory subunit alpha; protein kinase, cAMP-dependent, regulatory subunit type I alpha; protein kinase, cAMP-dependent, regulatory, type I, alpha; protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1); Tissue-specific extinguisher 1; TSE1 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG