CST, 5679S, Pdx1 (D59H3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDX1. Validated for WB,WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Pdx1 (D59H3) Rabbit Monoclonal Antibody (CST #5679) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 68797 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Pdx1 (D59H3) Rabbit Monoclonal Antibody detects endogenous levels of total Pdx1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val274 of human Pdx1 protein. Background The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (1,2). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (2,3). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (3), where it promotes the expression of genes important for β-cell functions such as insulin, glucokinase, and Glut2 (4-6). Mutations of the corresponding Pdx1 gene may be associated with diabetes and cases of pancreatic insufficiency (7). Alternate Names Glucose-sensitive factor; GSF; IDX-1; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF-1; IPF1; Islet/duodenum homeobox-1; IUF-1; IUF1; MODY4; PAGEN1; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1; STF1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG