CST, 5681S, IWS1 Antibody

Polyclonal Antibody for studying IWS1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, IWS1 Antibody (CST #5681) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity IWS1 Antibody detects endogenous levels of total IWS1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IWS1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Various steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation (1,2). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps (1,2). IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone (1,2). IWS1 also recruits another CTD-binding protein, HYPB/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation (2). Thus, IWS1 links Spt6 and HYPB/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level (2). Alternate Names DKFZp761G0123; FLJ10006; FLJ14655; FLJ32319; interacts with Spt6; interacts with SUPT6H, CTD assembly factor 1; IWS1; IWS1 homolog; IWS1 homolog (S. cerevisiae); IWS1-like protein; IWS1, SUPT6H interacting protein; IWS1L; MGC126375; MGC126376; Protein IWS1 homolog Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140 SOURCE: Rabbit