CST, 56832S, FNIP1 Antibody

Polyclonal Antibody for studying FNIP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, FNIP1 Antibody (CST #56832) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FNIP1 Antibody recognizes endogenous levels of total FNIP1 protein. A faint band detected at 130 kDa is predicted to be isoform 3. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys785 of human FNIP1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background FNIP1 and FNIP2 were identified as proteins interacting with tumor suppressor folliculin (FLCN) (1-3). FNIP1 and FNIP2 directly associate with AMPK, which indicates that they play roles in the energy and nutrient sensing pathways (1,2). Further studies show that the FLCN/FNIP2 complex functions as a GTPase-activating protein for Rag GTPase C and Rag GTPase D (4). The FLCN/FNIP2 complex thus promotes binding of mTORC1 to Rag heterodimers, leading to mTORC1 activation (4). In addition, along with FLCN, both FNIP1 and FNIP2 contribute to kidney tumor suppression (5). Alternate Names DKFZp686E18167; DKFZp781P0215; FNIP1; folliculin interacting protein 1; Folliculin-interacting protein 1; KIAA1961; MGC667 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit