CST, 5687T, PPP2R5D (H5D12) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PPP2R5D/PR61d. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PPP2R5D (H5D12) Mouse Monoclonal Antibody (CST #5687) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PPP2R5D (H5D12) Mouse Monoclonal Antibody recognizes endogenous levels of total PPP2R5D/PP2A, B'-δ/B56-δ protein. This antibody cross-reacts with PPP2R4 and PPP2R2A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PPP2R5D protein. Background Protein Phosphatase 2A subunit isoform R5-δ (PPP2R5D) belongs to the R5/B'/B56 family of the regulatory subunit B of serine/threonine phosphatase 2A (1). PPP2R5D (B-δ) is structurally related to the other components of subunit B, B-α. PPP2R5D lacks the WD-40 repeats that are important for protein-protein interaction (1-2). Structural differences may contribute to distinct down stream effects (3) and directing PP2A nuclear localization (4). Current evidence suggests that in contrast to B-α, PPP2R5D modulates the TGF-β/Actividin/Nodal signaling pathways by restricting the downstream activities of the type I TGF-β receptors, ALK4, and ALK5 (3) Alternate Names 2A5D; B56D; B56delta; MGC2134; MGC8949; MRD35; PP2A B subunit isoform B'-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta; PP2A, B subunit, B' delta isoform; PP2A, B subunit, B56 delta isoform; PP2A, B subunit, PR61 delta isoform; PP2A, B subunit, R5 delta isoform; PPP2R5D; protein phosphatase 2 regulatory subunit B'delta; protein phosphatase 2, regulatory subunit B (B56), delta isoform; protein phosphatase 2, regulatory subunit B', delta; protein phosphatase 2, regulatory subunit B', delta isoform; Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 66, 69 Source/Isotype: Mouse IgG1