CST, 5691S, LCMT1 (4A4) Mouse Monoclonal Antibody

Monoclonal Antibody for studying LCMT1 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, LCMT1 (4A4) Mouse Monoclonal Antibody (CST #5691) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LCMT1 (4A4) Mouse Monoclonal Antibody recognizes endogenous levels of total LCMT1 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to mouse LCMT1 protein. Background Leucine carboxyl methyltransferase-1 (LCMT1) is an important mammalian methyltransferase that methylates the catalytic C subunit of protein phosphatase 2A (PP2A) at Leu309 (1,2). X-ray crystallography analysis reveals that the structural integrity of human LCMT1 and cofactor S-adenosylmethionine complex enables proper interaction with PP2A (3). Evidence suggests that functional LCMT1 is crucial for the ability of PP2A to form heterotrimers with the B/B55 and Bα family of regulatory subunits (4,5). Impaired LCMT1 activity is shown to disrupt mitosis and cell survival (5). In neurodegenerative models, increases in endogenous levels of LCMT1, methylated PP2A (C), and Bα induce the development and differentiation of neuroblastoma cells (5-7). Alternate Names AL033290; Lcm; Lcmt; LCMT-; LCMT-1; Lcmt1; Leucine carboxyl methyltransferase 1; Leucine carboxyl methyltransferase 1, isoform CRA_b; MGC11807 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 39 Source/Isotype: Mouse IgG1