CST, 5696S, SMC3 (D47B5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMC3. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SMC3 (D47B5) Rabbit Monoclonal Antibody (CST #5696) is ready to ship. Product Usage Information Western Blotting: 1:2000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SMC3 (D47B5) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMC protein. In immunofluorescent analysis, this antibody shows nuclear staining as expected; however, it also shows weak cytoplasmic staining that is presumed to be background. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg132 of human SMC3 protein. Background The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Alternate Names BAM; Bamacan; Basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; Chondroitin sulfate proteoglycan 6; chondroitin sulfate proteoglycan 6 (bamacan); Chromosome-associated polypeptide; CSPG6; hCAP; SMC protein 3; SMC-3; SMC3; SMC3L1; structural maintenance of chromosomes 3; Structural maintenance of chromosomes protein 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG