CST, 5699S, IGBP1 (5F6) Mouse Monoclonal Antibody

Monoclonal Antibody for studying IGBP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IGBP1 (5F6) Mouse Monoclonal Antibody (CST #5699) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IGBP1 (5F6) Mouse Monoclonal Antibody recognizes endogenous levels of total IGBP1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human IGBP1. Background Immunoglobulin binding protein 1 (IGBP1) interacts with the regulatory subunit C of serine/threonine phosphatase PP2A, and other protein phosphotases, PP4 and PP6 (1-3). Binding of IGBP1 to PP2A has been shown to regulate PP2A catalytic activity and its substrate specificity (1-4). Recent evidence suggests that IGBP1 may play a role in PP2Ac ubiquitination via its association with E3 ubiquitin ligase MID1 (5,6). IGBP1 negatively regulates apoptosis by targeting PP2A activity to suppress p38 mitogen-activated protein kinase activation by cytokines (7). Upon BCR cross-linking, IGBP1 transiently associates with tyrosine phosphorylated molecules, which in turn induce downstream signal transduction (7). Evidence suggests that IGBP1 association with PP2A may be involved in the rapamycin sensitive mTOR pathway (8,9). Formation of the IGBP1 and Midline ring finger protein (MID) complex has been identified as a prerequisite to pathogenesis of X-linked Optiz GBBB syndrome (10). Alternate Names ALPHA-4; alpha4; B cell signal transduction molecule alpha 4; B-cell signal transduction molecule alpha 4; bA351K23.1 (immunoglobulin binding protein 1 (CD79A) ); CD79a-binding protein 1; IBP1; IGBP1; immunoglobulin (CD79A) binding protein 1; immunoglobulin binding protein 1; Immunoglobulin-binding protein 1; Protein alpha-4; Protein phosphatase 2/4/6 regulatory subunit; protein phosphatase 2A, regulatory subunit alpha-4; Renal carcinoma antigen NY-REN-16 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Mouse IgG1