CST, 57045S, USP30 (F3S4O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying USP30. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, USP30 (F3S4O) Rabbit Monoclonal Antibody (CST #57045) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity USP30 (F3S4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total USP30 protein. This antibody detects a 42 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu370 of human USP30 protein. Background Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes (1,2). Ubiquitin-specific-processing protease 30 (USP30), also known as ubiquitin carboxyl-terminal hydrolase 30, belongs to the USP subclass of DUBs. USP30 is located on the outer mitochondrial membrane, where it regulates mitochondrial dynamics and mitophagy by deubiquitinating outer mitochondrial membrane proteins, such as Tom20 and Miro1. Mitochondrial dysfunction is strongly implicated in the etiology of neurodegenerative diseases, including Parkinson's disease (PD). Knockdown of USP30 increases parkin-mediated mitophagy and clearance of dysfunctional mitochondria, making this protein a therapeutic target for PD. USP30 inhibition was shown to be protective in PD patient-derived neurons and in mouse models of PD, increasing mitophagy and decreasing oxidative stress, phosphorylated alpha-synuclein, and dopaminergic neuron loss. In addition to PD, USP30 is a therapeutic target for various cancers. Its expression is upregulated in many malignancies, while its inhibition has been shown to reduce cancer cell survival and proliferation (3-5). Alternate Names Deubiquitinating enzyme 30; FLJ40511; MGC10702; Ub-specific protease 30; Ubiquitin carboxyl-terminal hydrolase 30; ubiquitin specific peptidase 30; ubiquitin specific protease 30; Ubiquitin thioesterase 30; ubiquitin thiolesterase 30; ubiquitin-specific protease 30; Ubiquitin-specific-processing protease 30; UBP30; USP30 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 59 Source/Isotype: Rabbit IgG