CST, 57056S, Cathepsin K (E7U5N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying cathepsin K mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Cathepsin K (E7U5N) Rabbit Monoclonal Antibody (CST #57056) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 89623 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Cathepsin K (E7U5N) Rabbit Monoclonal Antibody recognizes endogenous levels of total cathepsin K protein. Non-specific focal cytoplasmic staining was observed in mouse testis by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly230 of mouse cathepsin K protein. Background Cathepsin K (CTSK) is a cysteine proteinase belonging to the cathepsin family, which all share a common papain-like structural fold and a conserved active site Cys-Asn-His triad of residues (1-3). Cathepsin K, initially synthesized as an inactive procathepsin K, is further processed into a mature enzyme, whose most studied function is to mediate bone resorption (1-6). Originally discovered in osteoclasts, its expression has also been associated with various cancers, such as prostate, breast, bone, lung, and renal carcinoma (6). Many pharmaceutical companies are working on the development of selective inhibitors for cathepsin K for anti-osteoporosis drugs (5). Alternate Names AI323530; cat; Cat K; Cathepsin K; catK; Ctsk; minisatellite 10q detected by probe MMS10; MMS10-Q; Ms10q Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 29, 43 Source/Isotype: Rabbit IgG