CST, 57058S, IL-18 (E8P5O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL18 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, IL-18 (E8P5O) Rabbit Monoclonal Antibody (CST #57058) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:5000 - 1:20000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 82990 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity IL-18 (E8P5O) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse IL-18 protein. Non-specific staining was observed in mouse stomach crypts by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant mouse IL-18 protein. Background Interleukin-18 (IL-18), originally known as interferon-gamma inducing factor (IGIF), is part of the IL-superfamily of cytokines (1,2). This proinflammatory cytokine is synthesized as an inactive precursor which requires cleavage by Caspase-1 to become an active, mature molecule, which is secreted by monocytes and macrophages (3,4,6). It induces IFNγ production in T-helper-1 (Th1) cells and natural killer (NK) cells, and can regulate innate immunity via both Th1 and Th2 responses (4,5). Elevated IL-18 levels are associated with autoimmune disease, but may be balanced by binding to IL-18 binding protein (IL-18BP), which along with IL-18 neutralizing antibodies, are being examined in clinical trials (6,7). Alternate Names IFN-gamma-inducing factor; Igif; IL 18; Il-; IL-1 gamma; IL-18; Il18; Interferon gamma-inducing factor; interferon-gamma-inducing factor; interleukin 18; Interleukin-1 gamma; Interleukin-18 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 17, 22 Source/Isotype: Rabbit IgG