CST, 5709S, ApoM (8F12) Mouse Monoclonal Antibody

Monoclonal Antibody for studying ApoM. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ApoM (8F12) Mouse Monoclonal Antibody (CST #5709) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ApoM (8F12) Mouse Monoclonal Antibody recognizes endogenous levels of both unglycosylated and glycosylated ApoM protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a purified recombinant fragment of human ApoM protein. Background Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2). Apolipoprotein M (ApoM) is a novel membrane bound apolipoprotein involved in lipid transport. ApoM primarily interacts with HDL and regulates its metabolism (3). It is highly expressed in hepatocytes and kidney proximal tubule cells. Recently, ApoM was shown to act as a vasculoprotective constituent of HDL by delivering S1P to the S1P receptor on endothelial cells (4). SNPs of ApoM have been associated with type 2 diabetes (5). Alternate Names alternative name: G3a, NG20; apo-M; Apolipoprotein M; APOM; G3a; HSPC336; NG20; NG20-like protein; Protein G3a Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 21, 25 Source/Isotype: Mouse IgG2a