CST, 57246S, PTBP1 (E4I3Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PTBP1. Validated for WB,IP,ChIP,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, PTBP1 (E4I3Q) Rabbit Monoclonal Antibody (CST #57246) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity PTBP1 (E4I3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTBP1 protein. This antibody does not cross-react with PTBP2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val10 of human PTBP protein. Background PTBP1 and PTBP2 are highly related pre-mRNA binding proteins that silence the splicing of alternative exons (1,2). While PTBP1 is widely expressed, PTBP2, also known as neural PTB or nPTB, is expressed in post-mitotic neurons. In other cell types, PTBP2 transcripts are alternatively spliced by PTBP1, which leads to nonsense-mediated decay (3). PTBP1 and 2 bind to similar regions across the transcriptome, but PTBP1 has a stronger repressive property likely due to cofactors such as Raver1 and Matrin3 (4-6). Overexpression of PTBPs in various cancers has been observed, leading to alternative splicing of key proteins in oncogenic pathways (6-9). Alternate Names 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; HNRNP-I; HNRNPI; HNRPI; MGC10830; MGC8461; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); polypyrimidine tract binding protein 1; Polypyrimidine tract-binding protein 1; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; PTBP1; RNA-binding protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 57, 59 Source/Isotype: Rabbit IgG