CST, 57248S, Mena (E8H6R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Mena. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Mena (E8H6R) Rabbit Monoclonal Antibody (CST #57248) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Mena (E8H6R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Mena protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Mena protein. Background Mena, EVL, and VASP are all members of the Ena/VASP family, which is involved in controlling cell shape and movement by shielding actin filaments from capping proteins (1). Ena/VASP proteins have three distinct domains: an amino-terminal EVH1 domain controlling protein localization, a central proline-rich domain mediating interactions with SH3 and WW domain-containing proteins, including profilin, and a carboxy-terminal domain that promotes tetramerization and actin-binding (2). Mena (also known as ENAH or Protein enabled homolog) interacts with actin filaments at the growing ends and is thus localized to lamellipodia and the tips of neuronal growth cone filopodia. Axons projecting from interhemispheric cortico-cortical neurons were shown to be misrouted in newborn, homozygous Mena knockout mice (3). Mena may be phosphorylated at Ser236 by PKA, a post-translational modification (PTM) that is reported to promote filopodial formation and elongation of the growth cone (4). Three forms of the Mena protein, with apparent molecular weights of 80, 88, and 140 kDa, have been described. The 80 kDa isoform is broadly expressed, whereas the 140 kDa isoform is reportedly enriched in neural cell types; these isoforms are generated by alternative splicing. The 88 kDa isoform is expressed primarily in embryonic cells and is likely the result of PTM of the 80 kDa isoform. Expression of all three forms is completely eliminated after homozygous deletion of , the gene encoding the Mena protein (1,3). Alternate Names ENA; enabled homolog; enabled homolog (Drosophila); ENAH; ENAH actin regulator; mammalian enabled variant 11a; mammalian enabled variant pan; MENA; NDPP1; Protein enabled homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80, 88, 140 Source/Isotype: Rabbit IgG