CST, 5724T, Phospho-Axl (Tyr702) (D12B2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Axl (Tyr702) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Axl (Tyr702) (D12B2) Rabbit Monoclonal Antibody (CST #5724) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 28451 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Axl (Tyr702) (D12B2) Rabbit Monoclonal Antibody detects endogenous levels of Axl only when phosphorylated at Tyr702. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases, such as EGFR. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr702 of human Axl protein. Background Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4). Phosphorylation of Axl Receptor on Tyr702 was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for phosphorylation site discovery (5). Phosphorylation of Axl on Tyr702 was also reported in select carcinoma cell lines (6). Alternate Names ARK; AXL; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; JTK11; oncogene AXL; Tyro7; Tyrosine-protein kinase receptor UFO; UFO Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 138 Source/Isotype: Rabbit IgG