CST, 5728T, ELP3 (D5H12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ELP3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ELP3 (D5H12) Rabbit Monoclonal Antibody (CST #5728) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ELP3 (D5H12) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELP3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly431 of human ELP3 protein. Background Elongator is a highly conserved transcription elongation factor complex that was first identified in yeast as part of the hyperphosphorylated RNA polymerase II (RNAPII) holoenzyme (1). The Elongator complex consists of 6 subunits, ELP1-6, and has been shown to have acetyltransferase activity (2). The acetylation targets of Elongator include histone H3, which is linked to the transcription elongation function of the complex, and α-tubulin, which is associated with regulation of migration and maturation of projection neurons (3-6). ELP3 is the catalytic acetyltransferase subunit of the Elongator complex (2,3). ELP3 contains an iron-sulfur cluster that can bind S-adenosylmethionine, which is necessary for the structural integrity of the Elongator and has been shown to play an important role in mediating global zygotic DNA demethylation of the paternal genome post-fertilization (7-9). Alternate Names elongation protein 3 homolog; elongation protein 3 homolog (S. cerevisiae); elongator acetyltransferase complex subunit 3; Elongator complex protein 3; ELP3; FLJ10422; hELP3; KAT9; Protein lysine acetyltransferase ELP3; tRNA uridine(34) acetyltransferase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG