CST, 57299S, R-Ras2 (F6T8H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RRas2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, R-Ras2 (F6T8H) Rabbit Monoclonal Antibody (CST #57299) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity R-Ras2 (F6T8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total R-Ras2 protein. This antibody does not cross-react with either R-Ras1 or R-Ras3 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human R-Ras2 protein. Background R-Ras2 (TC21) belongs to the R-Ras subfamily of small GTPases, which includes R-Ras1 and R-Ras3 (M-Ras). Similar to the classical Ras proteins, R-Ras2 is palmitoylated and farnesylated at its carboxy terminus, which facilitates association with membranes and potent activation of the ERK/MAPK and PI3K signaling cascades. Research studies have identified a role for R-Ras2 in the homeostasis of the adaptive immune system by demonstrating that loss of R-Ras2 expression impairs the proliferation and survival of lymphocytes in both the T cell and B cell compartments (1-3). R-Ras2 has also been implicated in human cancers. Indeed, overexpression of R-Ras2 has been observed in both lymphoid malignancies and solid tumor settings, concomitant with hyperactivation of signaling nodes that drive cell proliferation and survival (1,3-5). Alternate Names NS12; RAS related 2; Ras-like protein TC21; Ras-related protein R-Ras2; related RAS viral (r-ras) oncogene homolog 2; RRAS2; TC21; Teratocarcinoma oncogene Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG