CST, 57331S, Semaphorin-4A (E5N3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SEMA4A. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Semaphorin-4A (E5N3K) Rabbit Monoclonal Antibody (CST #57331) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 35176 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Semaphorin-4A (E5N3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total Semaphorin-4A protein. An unknown background band is detected at 55 kDa in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu756 of human Semaphorin-4A protein. Background Semaphorin-4A, or Sema4A, is a membrane-type class IV semaphorin family member glycoprotein and is expressed in dendritic cells, B cells, T cells, and endothelial cells (1). The protein structure consists of an N-terminus signal peptide, a sema domain, a C2 type Ig domain, a transmembrane region, and a cytoplasmic tail (2). Semaphorin-4A exerts diverse biological effects through several different receptor-mediated pathways and plays important roles in immunity, angiogenesis, neuronal migration, and tumor progression. Semaphorin-4A enhances T cell activation by interacting with TIM-2, and T cell-derived Semaphorin-4A plays a role in helper T cell (Th) differentiation (3,4). In regulatory T cells (Tregs), Semaphorin-4A plays an important role in enhancing stability through interactions with neuropilin-1 (Nrp1) (5). Semaphorin-4A interacts with Plexin D1, which is expressed in endothelial cells and negatively regulates angiogenesis (6). In presynaptic neurons, Semaphorin-4A promotes the development of inhibitory or excitatory synapses through interactions with Plexin B1 or Plexin B2, respectively (7). Semaphorin-4A has been identified as a therapeutic target to promote antitumor responses, and its expression in the tumor microenvironment (TME) has been suggested as a biomarker to predict response to immunotherapy (8). Alternate Names CORD10; FLJ12287; RP35; SEM4A; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4A; SEMA4A; SEMAB; semaphorin 4A; semaphorin B; Semaphorin-4A; Semaphorin-B; SEMB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG