CST, 5734S, C1QBP Antibody

Polyclonal Antibody for studying C1QBP. Validated for Western Blotting. Highly specific and rigorously validated in-house, C1QBP Antibody (CST #5734) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity C1QBP Antibody recognizes endogenous levels of total C1QBP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys220 of human C1QBP protein. Antibodies are purified by protein A and peptide affinity chromatography. Background C1QBP, also referred to as p32, p33, gC1q receptor (gC1qR), and hyaluronic acid binding protein 1 (HABP1), was originally identified via its binding interactions with Splicing Factor (SF-2) (1). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases (2), the tumor suppressor ARF (3-5), and multiple antigens of bacterial and viral origin (6). Research studies have shown that C1QBP is overexpressed in a number of cancer cell types (7), and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis (7). C1QBP has also been shown to inhibit the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress (8). Alternate Names ASF/SF2-associated protein p32; C1q globular domain-binding protein; C1QBP; complement C1q binding protein; Complement component 1 Q subcomponent-binding protein, mitochondrial; complement component 1, q subcomponent binding protein; COXPD33; gC1Q-R; GC1q-R protein; GC1QBP; gC1qR; Glycoprotein gC1qBP; HABP1; Hyaluronan-binding protein 1; Mitochondrial matrix protein p32; p32; p33; SF2AP32; SF2P32; splicing factor SF2-associated protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit