CST, 57376SF, Gelsolin (D9W8Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying gelsolin. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Gelsolin (D9W8Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #57376) is ready to ship. Product Usage Information This product is the carrier free version of product #12953. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12953 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Gelsolin (D9W8Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total gelsolin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val377 of human gelsolin protein. Background Gelsolin (actin-depolymerizing factor, ADF, AGEL, Brevin) is an 83 kDa protein that shares structural and functional homology to villin and adseverin/scinderin (1,2). Gelsolin plays an important role in actin filament assembly by capping and severing actin proteins in a Ca -dependent manner (3,4). Gelsolin is important for cellular events (e.g., membrane ruffling, chemotaxis, ciliogenesis) that require cytoskeletal remodeling (3). Accordingly, cells from gelsolin knockout mice exhibit motility defects, including a failure to ruffle in response to growth factor stimulation (5,6). In humans, defects in gelsolin have been linked to amyloidosis type 5 (AMYL5), a hereditary disease characterized by cranial neuropathy, which appears to result from gelsolin amyloid deposition (7). Alternate Names Actin-depolymerizing factor; ADF; AGEL; Brevin; DKFZp313L0718; epididymis secretory sperm binding protein; GELS; Gelsolin; gelsolin (amyloidosis, Finnish type); GSN Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 83 Source/Isotype: Rabbit IgG