CST, 57407S, RNMT Antibody

Polyclonal Antibody for studying RNMT. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, RNMT Antibody (CST #57407) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RNMT Antibody recognizes endogenous levels of total RNMT protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly40 of human RNMT protein. Antibodies are purified by peptide affinity chromatography. Background RNA guanine-7 methyltransferase (RNMT) is the enzyme responsible for providing the methyl group on the 7 position on the guanosine at the 5' cap of mRNAs. The methyl group provides a docking site for eIF4E and subsequent formation of the eIF4F complex to promote translation (1-4). RNMT binds along with activating subunit RNMT-activating miniprotein (RAM), which is required for efficient methylation of the mRNA cap (5). In addition to the methyltransferase activity, RNMT-RAM has been shown to affect RNA pol II occupancy, and knockdown has been shown to reduce mRNA production (6,7). RNMT-RAM has also been shown to play a role in rRNA synthesis by controlling RNA pol I activity through controlling expression of c-Myc (8). Alternate Names cm1p; CMT1; CMT1c; DKFZp686H1252; hcm1p; hCMT1; hCMT1c; hMet; KIAA0398; MCES; MET; mRNA (guanine-7-)methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap guanine-N7 methyltransferase; mRNA cap methyltransferase; RG7MT1; RNA (guanine-7-) methyltransferase; RNA guanine-7 methyltransferase; RNMT Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit