CST, 57481S, IL3RA/CD123 Antibody

Polyclonal Antibody for studying IL3RA. Validated for Western Blotting. Highly specific and rigorously validated in-house, IL3RA/CD123 Antibody (CST #57481) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting Specificity / Sensitivity IL3RA/CD123 Antibody recognizes endogenous levels of total IL3RA/CD123 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IL3RA/CD123 protein. Antibodies are purified by peptide affinity chromatography. Background IL3RA/CD123 is the alpha subunit of an IL-3 receptor that also contains the common beta chain (βc) shared with receptors for other cytokines. IL3RA/CD123 alone binds to IL-3 with high specificity but low affinity and it does not transduce intracellular signaling, while βc alone does not bind to IL-3 appreciably. Together, IL3RA/CD123 and βc form the functional high-affinity receptor for IL-3. IL3RA/CD123 is mainly expressed on the hematopoietic progenitor cells, primarily the granulocytic and monocytic linages (1,2). IL3RA/CD123 is highly expressed on leukemic stem cells in the majority of AML (acute myeloid leukemia) patients and is correlated with poor prognosis. In addition, overexpression of IL3RA/CD123 has also been reported for other hematopoietic malignancies (3-5). It is considered as a valuable therapeutic target for these malignancies (6-8). Alternate Names CD123; CD123 antigen; hIL-3Ra; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; IL3R; IL3RA; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor subunit alpha; interleukin 3 receptor, alpha (low affinity); Interleukin-3 receptor subunit alpha; MGC34174 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit