CST, 57518S, Myelin Protein Zero Antibody

Polyclonal Antibody for studying MPZ mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Myelin Protein Zero Antibody (CST #57518) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:1600 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Myelin Protein Zero Antibody recognizes endogenous levels of total Myelin Protein Zero protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val218 of mouse Myelin Protein Zero protein. Antibodies are purified by peptide affinity chromatography. Background Myelin Protein Zero (MPZ, myelin P0) is a single pass membrane glycoprotein member of the IgG superfamily (1,2). MPZ is the most abundant myelin protein in the peripheral nervous system and acts as a major structural component of the peripheral myelin sheath (2,3). It has been shown that MPZ is necessary for normal myelin spiraling, compaction, and maintenance, with disruption of the gene leading to hypomyelination and degradation of associated axons (4). Â Mutations in the gene have been associated with demyelinating peripheral neuropathies including Charcot-Marie-Tooth neuropathy type 1B (CMT1B), Dejerine-Sottas syndrome (DSS), and congenital hypomyelination (CH) (5,6). Alternate Names MPP; Mpz; myelin P0; Myelin peripheral protein; Myelin protein P0; Myelin protein zero; MYP0; OTTMUSP00000023997; OTTMUSP00000023998; P-zero; P0 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit