CST, 5754S, RCHY1 Antibody

Polyclonal Antibody for studying RCHY1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, RCHY1 Antibody (CST #5754) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RCHY1 Antibody detects endogenous levels of total RCHY1 protein. This antibody does not detect RCHY1 dimers. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RCHY1 protein isoform1. Antibodies are purified by protein A and peptide affinity chromatography. Background ING finger and zinc finger domain-containing protein 1 ( ) is a newly identified RING-H2-type protein-ubiquitin E3 ligase that is expressed as multiple isoforms generated through alternative splicing of mRNA transcripts (1,2). Notably, is a p53-regulated gene and multiple studies have shown that RCHY1 directly binds p53 to promote its ubiquitin-dependent proteasomal degradation, ultimately leading to repression of p53 growth suppressive transcriptional activity (1,3). RCHY1 is also likely to promote deregulated cell proliferation by targeting the CDK inhibitor, p27 Kip1, for proteasomal degradation (4). In agreement with its growth promoting activity, RCHY1 overexpression has been linked to cancer of the lung (5,6) and prostate (7). Alternate Names Androgen receptor N-terminal-interacting protein; androgen-receptor N-terminal-interacting protein; ARNIP; CH-rich interacting match with PLAG1; CH-rich-interacting match with PLAG1; CHIMP; DKFZp586C1620; E3 ubiquitin-protein ligase Pirh2; hARNIP; hPirh2; p53-induced protein with a RING-H2 domain; p53-induced RING-H2 protein; PIRH2; PRO1996; RCHY1; ring finger and CHY zinc finger domain containing 1; ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase; RING finger and CHY zinc finger domain-containing protein 1; RING finger protein 199; RING-type E3 ubiquitin transferase RCHY1; RNF199; ZCHY; Zinc finger protein 363; zinc finger, CHY-type; ZN363; ZNF363 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 29 SOURCE: Rabbit