CST, 57612S, FNIP2 (D3T8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FNIP2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FNIP2 (D3T8Z) Rabbit Monoclonal Antibody (CST #57612) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FNIP2 (D3T8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total FNIP2 protein. This antibody does not cross-react with FNIP1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly26 of human FNIP2 protein. Background FNIP1 and FNIP2 were identified as proteins interacting with tumor suppressor folliculin (FLCN) (1-3). FNIP1 and FNIP2 directly associate with AMPK, which indicates that they play roles in the energy and nutrient sensing pathways (1, 2). Further studies show that the FLCN/FNIP2 complex functions as a GTPase-activating protein for Rag GTPase C and Rag GTPase D (4). FLCN/FNIP2 complex thus promotes the binding of mTORC1 to Rag heterodimers, leading to the mTORC1 activation (4). In addition, along with FLCN, both FNIP1 and FNIP2 contribute to kidney tumor suppression (5). Alternate Names FNIP1-like protein; FNIP2; FNIPL; folliculin interacting protein 2; Folliculin-interacting protein 2; KIAA1450; MAPO1; O6-methylguanine-induced apoptosis 1 protein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG