CST, 57794S, EBF1 (E2I3Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EBF1. Validated for WB,WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, EBF1 (E2I3Q) Rabbit Monoclonal Antibody (CST #57794) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:1000 - 1:4000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 48836 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity EBF1 (E2I3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total EBF1 protein. This antibody may cross-react with EBF2 and EBF3. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly500 of human EBF1 protein. Background Early B-cell factor 1 (EBF1), also referred to as COE1, is a pioneering transcription factor that is crucial for defining B cell lineage. In hematopoiesis, EBF1 promotes the expression of genes that specify B cell lineage commitment, such as , , and , while also repressing the expression of transcription factor genes that promote alternate lineages (1,2). EBF1 has also been reported to play a role in olfactory neuronal cell, adipocyte, and early osteoclast differentiation (3-6). Due to the crucial role of EBF1 in B cell development, mutations in EBF1 can lead to the development of B-cell acute lymphoblastic leukemia (B-ALL) (7,8). EBF1 expression is also suppressed in some solid tumor cancers, including genomic loss in breast cancer and downregulation in pancreatic ductal adenocarcinomas (9,10). Lower expression levels of EBF1 also correlate with lower survival rates in patients with cholangiocarcinoma and colorectal carcinoma (CRC), and induced overexpression of EBF1 in CRC cell lines induces cell cycle arrest and apoptosis and (11,12). Alternate Names COE1; Collier, Olf and EBF transcription factor 1; early B cell factor 1; Early B-cell factor; early B-cell factor 1; EBF; EBF transcription factor 1; EBF1; FLJ39389; FLJ41763; O/E-1; OE-1; OLF1; olfactory neuronal transcription factor 1; Transcription factor COE1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG