CST, 57863SF, Myosin IIa (E7Y9O) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Myosin IIa. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Myosin IIa (E7Y9O) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #57863) is ready to ship. Product Usage Information This product is the carrier free version of product #49349. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 49349 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Myosin IIa (E7Y9O) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total myosin IIa protein. This antibody does not cross-react with myosin IIb or myosin IIc protein. This antibody cross-reacts with 20-25 kDa bands of unknown origin by western blot. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1954 of human myosin IIa protein. Background Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, , , and , which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7). Alternate Names BDPLT6; Cellular myosin heavy chain, type A; DFNA17; EPSTS; FTNS; MATINS; MGC104539; MHA; MYH9; MYH9 variant protein; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Myosin-9; myosin, heavy chain 9, non-muscle; NMHC-II-A; NMMHC II-a; NMMHC-A; NMMHC-IIA; NMMHCA; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 230 Source/Isotype: Rabbit IgG