CST, 57886S, LAMC1 (E9J7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAMC1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LAMC1 (E9J7Y) Rabbit Monoclonal Antibody (CST #57886) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LAMC1 (E9J7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMC1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly44 of human LAMC1 protein. Background Laminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of alpha (LAMA), beta (LAMB), and gamma (LAMC) chains. LAMC1 (laminin gamma-1) is a ubiquitously expressed gamma chain, and has three distinct functional domain structures. The N-terminal LN domain binds to specific alpha and beta chains to form a unique laminin heterotrimer. The LE domain mediates interactions with nidogens, while the C-terminal coil-coil fragment is necessary for agrin association. Collectively, these interactions are critical for stabilization and function of the BM (1). Genetic ablation of LAMC1 has demonstrated a critical role for this protein in embryo implantation, lung and kidney development, and neuronal Schwann cell myelination and regeneration, in addition to Trypanosome infection (2-5). Upregulation of LAMC1 has also been associated with tumor progression in multiple tumor types (6-9), possibly by creating a BM environment that is favorable for cancer cell metastasis and invasion. Alternate Names LAMB2; LAMC1; Laminin B2 chain; laminin subunit gamma 1; Laminin subunit gamma-1; Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; laminin, gamma 1 (formerly LAMB2); MGC87297; S-LAM gamma; S-laminin subunit gamma Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 220-250 Source/Isotype: Rabbit IgG