CST, 57893S, GPI Antibody

Polyclonal Antibody for studying G6PI. Validated for Western Blotting. Highly specific and rigorously validated in-house, GPI Antibody (CST #57893) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GPI Antibody recognizes endogenous levels of total G6PI protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro8 of human G6PI protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Glucose-6-phosphate isomerase (GPI) is a multi-functional protein belonging to the glucose phosphate isomerase family (1,2). As an intracellular metabolic enzyme, GPI plays a pivotal role in glycolysis and gluconeogenesis by catalyzing the interconversion of D-glucose-6-phosphate and D-fructose-6-phosphate (3). GPI is also secreted, where it functions as a cytokine (referred to as Autocrine Motility Factor, AMF), acting via the E3-ubiquitin-protein ligase AMFR/gp78 (4). In normal tissues, GPI/AMF has been shown to promote both immune cell maturation and neuronal cell survival (5,6). It is also secreted in abundance by some tumor cells (7), where it has been shown to promote tumor cell migration and metastasis (8,9). Alternate Names AMF; Autocrine motility factor; G6PI; glucose phosphate isomerase; Glucose-6-phosphate isomerase; GNPI; GPI; hexose monophosphate isomerase; hexosephosphate isomerase; Neuroleukin; NLK; oxoisomerase; PGI; PHI; Phosphoglucose isomerase; phosphohexomutase; Phosphohexose isomerase; phosphosaccharomutase; SA-36; SA36; Sperm antigen 36; sperm antigen-36 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit